Table 1. Treatment conditions during experiments.
Exp. | Dates | Nreplicates | Njar | Nshells | Salinity | Temp. | System pH | Jar pH | pCO2 * | Ωa * | TA | DIC | |
(psu) | (°C) | Durafet | Spec | Spec | (µatm) | (µmol/kg) | (µmol/kg) | ||||||
1 | 5/10–5/16 | 3 | 40 | 35 | 31.5 | 12.12±0.15 | 8.01±0.02 | 7.97 | – | 491 | 1.7 | 2239.6 | 2092.9 |
42 | 31.5 | 12.13±0.18 | 7.49±0.01 | 7.50 | – | 1576 | 0.62 | 2235.3 | 2236.5 | ||||
2 | 5/17–5/23 | 4 | 40 | 23 | 31.8 | 12.04±0.07 | 7.98±0.05 | 7.95±0.03 | 7.92 | 502±34 | 1.64±0.10 | 2237.8 | 2076.3 |
33 | 31.8 | 12.04±0.06 | 7.76±0.00 | 7.79±0.02 | 7.81 | 766±40 | 1.17±0.06 | 2239.2 | 2140.2 | ||||
23 | 31.8 | 12.05±0.07 | 7.48±0.01 | 7.46±0.03 | – | 1725±122 | 0.58±0.04 | 2230.5 | 2246.7 | ||||
3 | 6/29–7/5 | 4 | 33 | 46 | 31.9 | 12.08±0.08 | 8.02±0.00 | 7.97 | 7.99 | 462 | 1.50 | 2092.1 | 1940.5 |
26 | 31.9 | 12.07±0.08 | 7.47±0.01 | 7.46 | 7.46 | 1614 | 0.50 | 2085.6 | 2080.5 | ||||
4 | 31.9 | 12.09±0.09 | 7.19±0.01 | 7.22 | 7.21 | 2815 | 0.30 | 2084.5 | 2147.0 | ||||
4 | 7/3–7/9 | 2 | 39 | 13 | 29.6 | 12.09±0.09 | 8.02±0.00 | 7.98±0.00 | 7.97 | 460±3 | 1.51±0.01 | 2101.5 | 1943.6 |
14 | 29.6 | 12.08±0.07 | 7.47±0.00 | 7.47±0.02 | 7.47 | 1579±50 | 0.52±0.02 | 2097.4 | 2093.4 | ||||
0** | 29.6 | 12.11±0.12 | 7.19±0.01 | 7.19±0.05 | 7.12 | 3057±342 | 0.28±0.03 | 2092.2 | 2165.3 | ||||
5 | 7/6–7/12 | 3 | 40 | 24 | 29.7 | 12.12±0.13 | 8.02±0.00 | 7.97±0.00 | 7.96 | 460±4 | 1.53±0.01 | 2114.3 | 1956.6 |
21 | 29.7 | 12.09±0.09 | 7.47±0.00 | 7.47±0.02 | 7.46 | 1585±59 | 0.53±0.01 | 2108.1 | 2100.7 | ||||
7 | 29.7 | 12.12±0.12 | 7.18±0.01 | 7.14±0.02 | 7.15 | 3421±174 | 0.25±0.01 | 2101.7 | 2211.1 |
Exp. stands for experiment, temp. for temperature, spec. for spectrophotometer, TA for total alkalinity, and DIC for dissolved inorganic carbon. Nreplicates is the number of replicate jars in each treatment, Njar is the number of pteropods per jar, and Nshells is the number of shells in a treatment scored for shell condition.
* Ωa and pCO2 values calculated from DIC and pH measured via spectrophotometry.
** No pteropods survived this treatment in experiment 4.