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Western blot analysis of pERK1/2 in NSCs at different time points (0, 0.5, 1 or 2 h) after transfection of pcDNA3.1-IGF-1/GFP (A and B), and in IGF-1/GFP expressing NSCs treated with different concentrations of PD98059 (0, 5, 10, 20 or 40 µM) (C and D). *p<0.05 denotes as statistically significant in comparison to all other groups.
