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. 2014 Aug 27;9(8):e106010. doi: 10.1371/journal.pone.0106010

Figure 2. Upregulation of the LIF protein in zebrafish RGCs after optic nerve injury.

Figure 2

(A) As with the expression changes of LIF mRNA, the LIF protein was also upregulated starting on day 1 after the optic nerve injury, peaked at 3 days, and returned to baseline by 5 days post-injury (n = 3, *p<0.001). (B, C) Immunohistochemical analysis of the LIF protein expression revealed that LIF was upregulated mainly in GCL at 3 dpi (C) compared with the intact retina (B). (D–F) Double immunohistochemical staining of LIF (D) and Tuj1 (E) revealed that LIF is produced in RGCs (F). The scale bars in (B) and (D) are 30 µm.