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. 2014 Aug 27;9(8):e106010. doi: 10.1371/journal.pone.0106010

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© 2014 Ogai et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Effects of LIF morpholino (MO) on the expression of LIF mRNA at 3 dpi. LIF MO(1) decreased the amount of control (i.e., normal) LIF mRNA product (control; 571 bp) and increased the amount of LIF with intron 1 (+intron1; 833 bp). The asterisk denotes a non-specific product. (B) Effects of LIF MO on the expression of LIF protein at 3 dpi. As expected, LIF MO(2), along with LIF MO(1), decreased the amount of LIF protein compared with the control (ctrl.) MO treatment. (C–H) Immunohistochemical analysis of LIF after MO treatment at 3 dpi. All MOs are labeled with FITC. LIF MO clearly attenuated the expression of LIF in the RGCs (F–H) compared with the treatment with control MO (C–E). The scale bar in (C) is 30 µm.