Figure 4. Akt inhibition suppresses induction of Rictor by TGF-β.

Serum-starved IPF fibroblasts were pre-treated with Akti (Akt inhibitor VIII/124018) for 30 minutes or left untreated prior to TGF-β (5 ng/ml) treatment for two hours. In (A) cells were pre-treated with Akti at indicated concentration as shown, then followed by TGF-β treatment; (B) cells were pre-treated with Akti at 300 nM prior to TGF-β treatment or left untreated. Total cell lysates were prepared and equal amounts of protein were analyzed by Western blot analysis with specific antibodies as indicated. α-tubulin was used as a loading control.