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(A) Anti-PEG and (B) anti-human Hb antibody response in rats injected with HDAS-PEG2K-LEH or saline over 4 weeks (0, 7, 14, 21 days). Rat serum analyzed by ELISA. One group received weekly injections of HDAS-PEG2K-LEH at a dose rate of 10 ml/kg, whereas the other group was given equal volume of saline (control).
