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. 2014 May 5;16(9):1339–1353. doi: 10.1111/cmi.12290

Figure 3.

Figure 3

Inhibition of total cell GPX activity significantly augments transepithelial migration of PMNs. Intestinal epithelial cells maintained on transwells were treated with 2 mM of sodium iodoacetate (IOD; a pan GPX inhibitor) or HBSS+ (negative control) for 10 min, and then infected with S. Typhimurium SL1344 in the absence or presence of IOD, or treated with IOD alone or HBSS+. PMN transmigration was then performed and we show that inhibition of total cell GPX with IOD resulted in a significant increase in SL1344-induced PMN transmigration compared with SL1344 infection in the absence of IOD. (B). Cells were treated as above, supernatants were collected 4 h after infection, and lipids in the supernatants were semi-purified by column chromatography and eluted with methanol. Samples were dried, resuspended in 300 μl of HBSS+ and used to carry out 12S-HETE ELISA. Infection of cells with S. Typhimurium and IOD treatment resulted in a significant increase in 12S-HETE levels compared with uninfected cells (The symbols ‘*’, ‘**’ and ‘***’ indicate statistical significance, using the Student's t-test; P < 0.05, n = 3).