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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Cell Microbiol. 2014 May 1;16(9):1391–1404. doi: 10.1111/cmi.12299

Figure 3.

Figure 3

Effect of Cdt on downstream components of the PI-3K signaling pathway. Monocytes were treated with Cdt and then assessed for levels of PIP3 (panel A). Results are the mean ± S.E. for three experiments. The effect of Cdt on Akt and GSK3β phosphorylation is shown in panels B and C. THP-1 derived macrophages were exposed to 50 ng/ml of Cdt for 0–120 min then harvested, solubilized and fractionated by SDS-PAGE. Gels were analyzed by Western blot for the presence of Akt, pAkt(S473), GSK3β and pGSK3β(S9) followed by digital scanning densitometry; actin is shown as a loading control. A representative Western blot is shown in panel B and the mean ± SEM is shown for three experiments in panel C. Asterisks indicate p<0.05 when compared to untreated cells.