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Cdt induces macrophages to release pro-inflammatory cytokines. THP-1-derived macrophages (hatched bars) and MDM (solid bars) were treated with Cdt (0–200 ng/ml) and the supernatants analyzed 5 hr (THP-1) or 24 hr (MDM) later by ELISA for IL-1β (panel A), TNFα (panel B) and IL-6 (panel C). Results are the mean ± SEM for three experiments each performed in triplicate; asterisks indicate p<0.05 when compared to untreated cells.
