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Effect of CdtC CRAC mutant on cytokine release. THP-1-derived macrophages were treated with CdtABCWT or CdtABCY71P for 5 hr. Supernatants were analyzed by ELISA for IL-1β and TNFα. Results are the mean ± SEM for three experiments each performed in triplicate; asterisk indicates statistical significance (p<0.05) when compared to CdtABCWT at the same concentration.
