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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Dev Comp Immunol. 2014 Jun 28;47(1):52–58. doi: 10.1016/j.dci.2014.06.012

FIG 5.

FIG 5

Quantitative gene expression analysis by qRT-PCR in 1-year old X. laevis adult kidneys for TNF-α, Type I IFN, Mx1 6 days after i.p. injection with either FV3 or mock-infection with APBS. Results are presented as means ± SEM from mock-infected (N=3/group) and infected frogs (N=4–5/group). Statistical significance (ANOVA): (*) P<0.05 between non-infected and infected sham-atrazine exposed controls. There was no statistical difference between atrazine exposed and sham-exposed infected groups. Gene expression is represented as fold increase (RQ: relative quantification) relative to GAPDH endogenous control and standardized to uninfected non-atrazine exposed controls (fixed as 1).