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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Cell Microbiol. 2014 Jun 2;16(9):1441–1455. doi: 10.1111/cmi.12305

Fig. 3. The β1 integrin is required for full activation of Erk 1/2 and IL-8 gene transcription in response to C. jejuni.

Fig. 3

Panels: (A) INT 407 cells were transfected with siRNA specific for the β1 integrin or scramble (Scrm) siRNA. The cells were infected with C. jejuni and incubated for 30 min. Uninfected cells served as a negative control. The cell lysates were analyzed by immunoblot using antibody against pErk 1/2. The membranes were re-probed using antibody against actin as a loading control. Densitometric analysis was performed to quantify the level of Erk 1/2 activation. (B) INT 407 cells were treated with siRNA and infected with C. jejuni. The level of IL-8 gene expression following a 6 hr infection was measured by RT-PCR. IL-8 expression was normalized to GAPDH transcript levels with uninfected cells serving as negative control.