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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Cell Microbiol. 2014 Jun 2;16(9):1441–1455. doi: 10.1111/cmi.12305

Fig. 5. FAK is critical to Erk 1/2 activation and IL-8 secretion in response to C. jejuni.

Fig. 5

Panels: (A) INT 407 cells were treated with focal adhesion kinase (FAK) inhibitor TAE 226 and infected with C. jejuni. Immunoblots coupled with densitometric analysis were used to determine the level of Erk 1/2 activation following a 40 min incubation. (B) INT 407 cells were treated with TAE 226 and infected with C. jejuni. The level of IL-8 gene expression was measured by RT-PCR following a 6 hr infection. IL-8 expression was normalized to GAPDH transcript levels with uninfected cells serving as a negative control. (C) INT 407 cells were treated with TAE 226 and infected with C. jejuni. Supernatants were collected after 6 hr and IL-8 was quantified by ELISA. Multiple TAE 226 concentrations were tested to determine if there was a dose-dependent effect. Uninfected cells served as a negative control. (D) Caco-2 human intestinal epithelial cells were treated with TAE 226 and infected with C. jejuni. Supernatants were collected after 6 hr incubation, and IL-8 was quantified by ELISA. Uninfected cells served as negative control.