Figure 4. Macromolecular synthesis in the presence of increasing anthracimycin.

Incorporation of radiolabeled precursors of DNA, RNA, protein and cell wall synthesis ([³H]-Thymidine, [³H]-Uridine, [³H]-Leucine, and [³H]-N-Acetylglucosamine, respectively) was measured using S. aureus ATCC29213 (MSSA). DNA intercalation was examined using a commercially available kit. In the absence of DNA intercalation, relaxed plasmid migrates as a series of discrete topomers, with a characteristic ladder pattern. In contrast, intercalation results in a change in linking number, resulting in a change in the migration pattern. “SC” = supercoiled; “Rel” = relaxed.