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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Arthritis Rheumatol. 2014 Sep;66(9):2482–2493. doi: 10.1002/art.38723

Figure 5.

Figure 5

MIF treatment did not alter Lyn expression in BMM from CD74 KO. (A) BMM from WT and CD74KO were cultured with M-CSF and RANKL (both 30 ng/ml) with or without MIF (25 ng/ml) for 3, 4 or 5 days. The equal amount of whole cell protein from BMM cultures was immunoblotted for Lyn, c-Src, and CD74. (B) BMM cells from CD74KO mice were cultured with M-CSF alone for 3 days before treated with RANKL and/or MIF for up to 30 min. The equal amount of whole cell protein from cultures was immunoblotted for Syk, PLCγ, Gab2, JNK1 and c-jun antibodies. β-actin was used as control. (C) Microphotographs of bone marrow (BM) or bone marrow macrophage (BMM) cells from WT or LynKO mice were cultured with or without MIF (25 ng/ml) in the presence of M-CSF and RANKL (both at 30 ng/ml) for 5 days. BM (upper panels) or BMM (lower panels) cells from LynKO mice formed increased number of TRAP(+) OCL compared to WT cells. In addition MIF treatment did not alter TRAP(+) OCL formation in cells from LynKO mice. (D) Number of TRAP(+) OCL/well was scored. (E) BMM cells from WT or LynKO mice were tested for MIF responsiveness by immunoblotting. BMM cells were stimulated with or without MIF for up to 30 minutes in the presence of M-CSF and RANKL. B-actin was used as control. Values represent mean±SEM. *, Significant effect of MIF treatment, p<0.05. #, Significant effect of LynKO mice, p<0.05.