Table 1. Primers for real-time PCR gene quantitation.
Gene | Protein | Forward primer, 5’→3’ | Reverse primer, 5’→3’ |
---|---|---|---|
Dusp4, Mkp2 |
DUSP4 | ATTCCGCCGTCATCGTCTAC | ATAGCCACCTTTCAGCAGGC |
Nos3 | NOS3 | TACCAGCCGGGGGACCACATAGGC | CTCCAGCTGCTCCACAGCCACAGAC |
Gch1 | GCH1 | GCCATGCAGTTCTTCACCAAA | CCGATATGGACCTTTCCAACAAA |
Actb | β-actin | TGCCCATCTATGAGGGGTACG | GGACGATTTCCGCTCGGC |