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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Hepatology. 2014 Jul 25;60(3):872–883. doi: 10.1002/hep.27200

Figure 3.

Figure 3

AFP mediates the down regulation of miR-29a through c-MYC. A) AFP is over expressed and c-MYC silenced 72 hours post-transfection in HLE cells. B) miR-29a expression is only silenced when AFP is present and c-MYC is functionally active. Upon c-MYC silencing, miR-29a expression is induced even in the presence of AFP. C) The miR-29a/b-1 transcript is shown with two sets of primers designed around c-MYC binding sites. P-1 is in the promoter region and P-2 located in the intron of the transcript. Transcript schematic is modified from that created by Chang et al (22). D) Chromatin Immunoprecipitation was performed to pull down c-MYC bound to genomic regions in HLE cells after AFP+ or AFP− CM was applied (rabbit IgG was used as a control). Both P-1 and P-2 primer sets were used to quantify the amount of c-MYC bound to two specified regions on the miR-29a transcript using qRT-PCR. Results show that c-MYC bound to both regions of miR-29a/b-1 only in the presence of AFP.