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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Liver Int. 2014 Feb 7;35(4):1133–1144. doi: 10.1111/liv.12456

Figure 5.

Figure 5

Quantification of genes involved in lipogenesis. A. SREBP-1c protein expression was measured by western blot. Decreased concentration of high molecular weight (pre) SREBP1-c, results in increased lower molecular weight (nuclear) SREBP1-c, indicating SREBP1-c proteolytic activation. B. Densitometric quantification of Western blots was performed using Adobe Photoshop CS3, showing an increased SREBP1-c activation in ΔL Fxr animal under control diet. RTPCR was performed on RNA samples isolated from liver of flox/flox, ΔL Fxr and ΔIE Fxr knockout mice for C. Fas, D. Scd-1 and E. Pnpla3. Data represent means ± SEM (n = 4-5). Asterisk represents P<0.05 as determined by the Mann-Whitney U-test.