Table 1.
Information of the identified proteins in human hepatocarcinoma cell line SMMC-7721
| No. | NCBI/SWISS-PROT accession No. | Protein description | Theoretical pI/Mr (kDa) | Experimental pI/Mr (kDa) | Profound score | Normal volume |
| 159 | P38646 | HSP70, heat shock 70-ku protein 9B precursor | 5.9/73.7 | 5.4/71.0 | 2.37 | 1.228 |
| 149 | P38646 | HSP70, heat shock 70-ku protein 9B precursor | 5.9/73.7 | 5.4/71.8 | 2.30 | - |
| 259 | P10809 | Chaperonin CH60, | 5.7/61.2 | 5.3/58.7 | 2.33 | 0.271 |
| 60-ku heat-shock protein, mitochondrial precursor | ||||||
| 136 | P11021 | GRP78, Bip, 78-ku glucose-regulated protein precursor | 5.2/71.0 | 5.1/75.4 | 2.37 | - |
| 275 | P27797 | Calreticulin precursor | 4.3/48.2 | 4.4/56.0 | 2.35 | 0.383 |
| 757 | P30040 | Endoplasmic reticulum protein ERp29 precursor (ERp31) (ERp28) | 6.8/29.0 | 6.0/29.3 | 2.26 | 0.374 |
| 278 | P30101 | Protein disulfide isomerase ER60 precursor | 6.0/57.1 | 5.7/55.6 | 2.42 | 0.958 |
| 265 | P07237 | Protein disulfide isomerase precursor | 4.8/57.0 | 4.8/57.7 | 2.41 | 0.734 |
| (Prolyl 4-hydroxylase, beta subunit) | ||||||
| 789 | Q13162 | Peroxiredoxin-4 (Prx-IV) (Thioredoxin peroxidase AO372) (Thioredoxin-dependent peroxide reductase A0372) (antioxidant enzyme AOE372) | 5.9/30.7 | 5.6/28.5 | 2.38 | 0.785 |
| 849 | 32483377 | (NP_054817.2) Peroxiredoxin 3 isoform b; thioredoxin-dependent peroxide reductase precursor | 7.1/26.1 | 6.0/26.5 | 1.73 | 1.141 |
| 645 | P21796 | Voltage-dependent anion channel protein I (VDAC1) (plasmalemmalporin) (outer mitochondrial membrane protein porin) (PORIN 31NL) (porin 31HM) | 8.8/30.8 | 9.2/34.4 | 2.23 | 1.572 |
| 372 | P31930 | Ubiquinol-cytochrome C reductase complex core protein I | 5.9/53.3 | 5.5/45.5 | 2.29 | 0.121 |
| 699 | Q13011 | Peroxisomal enoyl-CoA hydratase | 6.6/36.1 | 6.0/32.3 | 2.14 | 0.927 |
| 319 | P06576 | ATP synthase beta chain, mitochondrial precursor | 5.3/56.6 | 5.1/51.1 | 2.39 | 0.222 |
| 774 | P30084 | Mitochondrial short-chain enoyl-coenzyme A hydratase 1 | 6.9/32.0 | 5.9/29.0 | 2.26 | 0.773 |
| 295 | P55809 | Probable 3-oxoacid CoA transferase 1 precursor | 7.2/56.6 | 6.0/53.5 | 1.65 | 0.449 |
| 108 | Q16891 | Mitochondrial inner membrane protein (Mitofilin) (p87/89) | 6.1/84.0 | 5.8/81.8 | 2.24 | 0.410 |
| 714 | P35232 | Prohibitin | 5.6/29.9 | 5.5/31.1 | 2.18 | 1.923 |
| 397 | P08670 | Vimentin | 4.8/41.7 | 4.8/43.2 | 2.19 | - |
| 283 | P07437 | Tubulin beta-2 chain | 4.7/50.2 | 5.0/55.0 | 2.37 | - |
| 153 | P02545 | Lamin A/C isoform 1 precursor | 6.6/74.0 | 6.2/71.6 | 2.30 | 0.297 |
| 150 | P02545 | Lamin A/C isoform 2 | 6.4/65.1 | 6.1/71.8 | 2.28 | 0.444 |
| 246 | P02545 | Lamin A | 6.4/57.7 | 6.2/60.4 | 1.80 | 0.16 |
| 415 | P02570 | Actin cytoplasmic 1 | 5.3/41.7 | 5.3/42.4 | 2.39 | - |
| 416 | P02570 | Actin cytoplasmic 1 | 5.3/41.7 | 5.4/42.6 | 2.23 | - |
| 417 | P02570 | Actin cytoplasmic 1 | 5.3/41.7 | 5.4/42.7 | 2.18 | - |