Figure 4.

RT-PCR analysis of purified LECs. RT-PCR was assessed on mRNA extracted from purified LECs at the 3rd passage. Data shown are agarose gel electrophoresis of amplification products. Isolated human liver cells (h-hep) and HepG2 cells were used as positive controls. LECs expressed human fetal liver markers (α-fetoprotein (AFP) and transcription factor Oct-4) and adopted hepatocyte (positive for ALB, CK-8, G6P, AAT, and CYP1B1) and biliary (positive for CK-19, GGT and MRP2) phenotypes. These cells did not express HNF4, TAT, CYP2B6 and CYP3A4.