Table 2.
Primers used for RT- PCR analysis of LECs
| Gene name | Primers | PCR product size |
| AFP | 5’-TGAAATGACTCCAGTAAACCC-3’ | 199 pb |
| 5’-GATGAAGCAAGAGTTTCTCATT-3’ | ||
| Oct-4 | 5’-CGA CCA TCT GCC GCT TTG AG-3’ | 573 pb |
| 5’-CCC CCT GTC CCC CAT TCC TA-3’ | ||
| Human hepatocyte nuclear factor 4 (HNF4) | 5’-CCA AGT ACA TCC CAG CTT TC-3’ | 295 pb |
| 5’-TTG GCA TCT GGG TCA AAG-3’ | ||
| ALB | 5’-CCTTGGTGTTGATTGCCTTTGCTC-3’ | 308 pb |
| 5’-CATCACATCAACCTCTGGTCTCACC-3’ | ||
| CK-8 | 5’-AAG GGC TGA CCG ACGAGA TC-3’ | 537 pb |
| 5’-GCT TCC TGT AGG TGG CGA TC-3’ | ||
| Glucose 6 phosphatase (G6P) | 5’-TCA GCT CAG GTG GTC CTC TT-3’ | 291 pb |
| 5’-CCT CCT TAG GCA GCC TTC TT-3’ | ||
| α-antitrypsin (AAT) | 5’-TCGCTACAGCCTTTGCAATG-3’ | 142 pb |
| 5’-GGAACTCCTCCGTACCCTCAA-3’ | ||
| Tyrosine aminotran- sferase (TAT) | 5’-TGA GCA GTC TGT CCA CTG CCT-3’ | 359 pb |
| 5’-ATG TGA ATG AGG AGG ATC TGA G-3’ | ||
| Cytochrome P450 1B1 (CYP1B1) | 5’-GAGAACGTACCGGCCACTATCACT-3’ | 357 pb |
| 5’-GTTAGGCCACTTCACTGGGTCATGAT-3’ | ||
| CYP2B6 | 5’-CCT CTT CCA GTC CAT TAC CG-3’ | 551 pb |
| 5’-TGA CTG CCT CTG TGT ATG GC-3’ | ||
| CYP3A4 | 5’-TGC TGT CTC CAA CCT TCA CC-3’ | 802 pb |
| 5’-TAG CTT GGA ATC ATC ACC ACC-3’ | ||
| CK-19 | 5’-TTTGAGACGGAACAGGCTCT-3’ | 426 pb |
| 5’-CAGCTCAATCTCAAGACCCTG-3’ | ||
| GGT | 5’-GAC GAC TTC AGC TCT CCC AG-3’ | 489 pb |
| 5’-CTT GTC CCT GGA TTG CTT GT-3’ | ||
| Multidrug resistance- associated protein 2 (MRP2) | 5’-ACA CCA ACC AGA AAT GTG TC-3’ | 660 pb |
| 5’-CCA AGG CCT TCC AAA TCT C-3’ | ||
| Glyceraldehyde- 3-phosphate dehydrogenase (GAPDH) | 5’-CGGAGTCAACGGATTTGGTCGTAT-3’ | 307 pb |
| 5’-AGCCTTCTCCATGGTGGTGAAGAC-3’ |