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A: Western blot analysis of MEK1/2 and Akt phosphorylation in CXCL12-treated cholangiocarcinoma cells. RMCCA1 and KKU 100 were treated with 100 ng/mL of CXCL12 for indicated time. MEK1/2 and Akt phosphorylation were determined by Western blot as described; B: The average band intensity based on 3 biologically separate experiments, showing levels of MEK1/2 and Akt phosphorylation relative to the levels of MEK1/2 and Akt expression from the same experiment (aP < 0.05).
