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. 2007 Feb 28;13(8):1187–1194. doi: 10.3748/wjg.v13.i8.1187

Figure 1.

Figure 1

Analysis of p16 (A) and MGMT (B) gene methylation by MSP. The presence of a visible PCR product in lanes U indicates the presence of unmethylated genes of p16 (151 bp) and MGMT (93 bp); the presence of product in lanes M indicates the presence of methylated genes of p16 (150 bp) and MGMT (81 bp). The samples of colorectal carcinoma 1, 2 and 4 show p16 promoter hypermethylation, while 6, 8 and 9 show MGMT promoter hypermethylation. NL-normal lymphocytes as a positive control for unmethylated alleles; Raji-commercial cell line as a positive control for methylated alleles; St-molecular weight marker (50 bp).