Table 1.

Sequences of amplification and sequencing primers

Forward primers		Positions nt
E1	5' GAGGGGGCTGTGCAGTGGATG 3'	6057-6077
I3	5' TCCGGCTCGTGGCTAAGGGA 3'	6246-6265
S2	5' GGATTTCCACTACGTGACGGG 3'	6620-6640
S4	5' GGGTCTCCCCCCTCCTTGGCC 3'	6906-6926
S31	5' GGACTACGTCCCTCCGGTGG 3'	7241-7260
Reverse primers
AS2	5' CGTCACGTAGTGGAAATCCCC 3'	6618-6638
AS32	5' GGGACGTAGTCCGGGTCCTTC 3'	7232-7252
I4	5' GCAGCAGACGAGATCCTCAC 3'	7567-7586
E2	5' GCTGCGAGATGTTGTGGCGTA 3'	7698-7718
Cloning primers	(pMOS vector)
SeqS1	5' GGGAAAGCTTGCATGCCTGC 3'
SeqAS2	5' GACGTTGTAAAACGACGGCC 3'

Nucleotide positions are numbered according to the HCV-J sequence and the NS5A region is within positions 6246-7586.