Fig. 4.

Comparative quantitative RT-PCR analysis of the relative expression of AlHSP90 and analysis of nymph’s mortality after culturing A. lucorum nymphs at different temperatures. a Relative AlHSP90 mRNA expression of A. lucorum nymphs (first to fifth instars) reared at six different temperatures. b The mortality rate of A. lucorum nymphs (first to fifth instars) reared at six different temperatures. A. lucorum insects reared at 24 °C were set up as controls. Different letters above each bar indicate statistical difference with a statistical analysis system (SAS) followed by Duncan’s multiple comparison test (p < 0.01)