Fig. 5.

Comparative quantitative RT-PCR analysis of the relative expression of AlHSP90 after A. lucorum female (male) adults cultured at different temperatures. a Relative AlHSP90 mRNA expression of female A. lucorum adults (new emergence, pre-mating, and post-mating stages) reared at six different temperatures. b Relative AlHSP90 mRNA expression of male A. lucorum adults (new emergence, pre-mating, and post-mating stages) reared at six different temperatures; A. lucorum insects reared at 24 °C were set up as controls. Different letters above each bar indicate statistical difference with a statistical analysis system (SAS) followed by Duncan’s multiple comparison test (p < 0.01)