Fig. 8.

Comparative quantitative RT-PCR analysis of the relative expression of AlHSP90 after treating 2-day-old female (male) A. lucorum adults with four different pesticides and heat shock (33 °C). Eb, Cy, Ch, and Im are 2-day-old A. lucorum adults treated with cyhalothrin, imidacloprid, chlorpyrifos, and emamectin benzoate (induction dose = LD₅₀); Eb-t, Cy-t, Ch-t, and Im-t are the 2-day-old A. lucorum adults treated with four different pesticides (cyhalothrin, imidacloprid, chlorpyrifos, and emamectin benzoate (induction dose = LD₅₀)) and heat shock (33 °C) at the same time. Ct are the same batches of A. lucorum adults reared at 24 °C, set up as the controls; Ct-t are the Ct control A. lucorum adults treated with heat shock (33 °C). Ac are the Ct control A. lucorum adults treated with acetone; Ac-t are the Ct control A. lucorum adults treated with both acetone and heat shock (33 °C) at the same time. Different letters above each bar indicate statistical difference with a statistical analysis system (SAS) followed by Duncan’s multiple comparison test (p < 0.01)