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. 2014 Feb 13;19(5):705–713. doi: 10.1007/s12192-014-0498-3

Fig. 5.

Fig. 5

The protective effects of adiponectin against cytotoxicity of HUVECs under Ang II treatment require binding of adiponectin to its cell surface receptors, AdipoR1 or AdipoR2. NS non-specific RNA; siAdipoR1 small RNA interference of AdipoR1; siAdipoR2 small RNA interference of AdipoR2. a Expression of adiponectin receptor 1 (AdipoR1) was knocked down using small RNA interferences, and western blot results confirmed successful knockdown (*p < 0.01 vs. NS group, n = 4); b LDH release experiment revealed that the protective effects of adiponectin against Ang II cytotoxicity were lost in cells with knockdown of AdipoR1 (ANOVA *p < 0.001 vs. non-treated control, n = 4–5); c Expression of adiponectin receptor 2 (AdipoR2) was knocked down using small RNA interferences, and western blot results confirmed successful knockdown (*p < 0.01 vs. NS group, n = 4); d LDH release experiments revealed that the protective effects of adiponectin against Ang II cytotoxicity were not lost in cells with knockdown of AdipoR2 (ANOVA *p < 0.001 vs. non-treated control; #p < 0.001 vs. Ang II-treated group, n = 4–5)