Figure 2.

Nuclear morphology of sperm head after injection into the MII stage sheep oocytes stained with Hoechst, 16 hr after ICSI: A) An oocyte with two polar bodies (pb), one female pronucleus (pn), and one sperm head (sh) suggesting oocyte activation after ICSI; B) Oocyte with swollen sperm head; C) Oocyte with metaphase plate (mp); D) Oocyte with two metaphase plates; E) Oocytes with two pronuclei and without sperm head suggesting normal fertilization; F) Oocyte with three pronuclei suggesting abnormal fertilization