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. 2014 Jul-Sep;6(3):147–155.

Figure 1.

Figure 1

Optimization of PEI amount. Varying amounts of PEI were added as indicated with a constant DNA amount of 1.25 µg/106 cells and a starting cell density of 2×106cells/ml. A) GFP positive cells 48 hr posttransfection with different PEI concentrations; B) t-PA recombinant protein concentration in the culture media measured on day 9 posttransfection by ELISA