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Optimization of the starting cell density for transfection. Varying starting cell densities from 0.2 to 4×106cells/ml with constant DNA and PEI concentrations were investigated. A) GFP positive cells 48 hr posttransfection with different starting cell densities; B) t-PA recombinant protein concentration in the culture media measured on day 9 posttransfection by ELISA
