Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of three different peptones on transfection efficiency and production yield. 1 g/l of final concentrations of peptones were added to the transfection reaction with 0.5 µg/106 cells of DNA, 0.5×106 starting cell density and 1.5 µg/ 106 cells of PEI concentrations. A) GFP positive cells 48 hr posttransfection with different peptone feeding strategies; B) t-PA recombinant protein concentration in the culture media measured on day 9 posttransfection by ELISA
