Table 2.

Specific activity and kinetics parameters of wild-type enzyme and mutant enzymes.

	WT	V94W	I100F
Specific activity (U mg−1)	228.42 ± 5.17	171.20 ± 4.06	166.98 ± 5.48
K m (mmol)	0.106 ± 0.003	0.128 ± 0.002	0.118 ± 0.007
V max⁡ (μmol min−1)	0.0135 ± 0.0009	0.0092 ± 0.0006	0.0089 ± 0.0006
k cat (s−1)	157 ± 1	107 ± 1	104 ± 1
k cat/K m (mmol−1 s−1)	1491 ± 31	835 ± 16	889 ± 43

∗The activities were tested in 50 mmol pH 8.0 PBS at 40°C. The values represent the means of three independent experiments (mean ± SD).