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. 2014 Jun 16;5(12):3983–3995. doi: 10.18632/oncotarget.2108

Fig 2. Biological compartment masking and biomarker example image panel.

Fig 2

(A) Representative image examples from each fluorescence channel used to generate architectural compartment masks during AQUA analysis. DAPI was used to generate a nuclear mask, cytokeratin (FITC) to identify tumor membrane/cytoplasm, vimentin (Cy3) to identify stroma, CD31 (Cy7) was used to identify vasculature, and in conjunction with cytokeratin to determine tumor vasculature and with vimentin to identify stromal vasculature. (B) Representative image examples of the TEM-1 associated biomarker panel (Cy5).