Figure 2. Expression of miRNAs.

(a) Length distribution of small RNAs in L1- active and L1-silenced cells. (b) Scatter plot showing normalized read counts for miRNAs in L1-silenced cells versus L1-active. Blue symbols denote miRNAs. (c) Bland-Altman plot of normalized read counts for miRNAs in L1-silenced library versus L1-active. The difference in the read counts (L1-silenced – L1-active) (vertical axis) is plotted against the average of the read counts [(L1-silenced + L1-active)/2]. If the read count for a particular miRNA species is identical in both libraries, the point is located on the middle horizontal line. The other horizontal lines represent the ± 2SD thresholds for the data. Points lying further away from the middle horizontal line are more likely to be significantly differentially expressed. (d) Higher magnification view of left-hand side of Figure 2c.