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. 2014 May 20;5(12):4257–4268. doi: 10.18632/oncotarget.1991

Figure 1. Up-regulation of HDAC2 expression promotes survival of p53 wild type neuroblastoma cells.

Figure 1

A and B, Kelly and SK-N-BE(2) neuroblastoma cells were transfected with scrambled control siRNA, N-Myc siRNA-1, N-Myc siRNA-2, HDAC2 siRNA-1 or HDAC2 siRNA-2 for 48 hours. RNA and protein were extracted from the cells and subjected to RT-PCR (A) and immunoblot (B) analyses of N-Myc and HDAC2 mRNA and protein expression. C, Kelly and SK-N-BE(2) cells were transfected with control siRNA, HDAC2 siRNA-1 or HDAC2 siRNA-2 for 72 hours. Cells were then stained with FITC-conjugated Annexin V and subjected to flow cytometry studies. The percentage of cells positively stained with Annexin V was analysed using FlowJo software. Error bars represent standard error. ** indicates P < 0.01 and *** indicates P < 0.001.