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. 2014 May 20;5(12):4257–4268. doi: 10.18632/oncotarget.1991

Figure 3. N-Myc and HDAC2 block p53 protein phosphorylation at Ser-46.

Figure 3

A and B, Kelly neuroblastoma cells were transfected with control siRNA, N-Myc siRNA-1, N-Myc siRNA-2, HDAC2 siRNA-1 or HDAC2 siRNA-2 for 48 hours. A, protein was extracted from the cells and subjected to immunoblot analyses with anti-Ser-46-phosphorylated p53 (Ser-46-phos p53), anti-total p53 and anti-TP53INP1 antibodies. B, the ratio of Ser-46-phos p53 to total p53 was quantified. C, Kelly and SK-N-BE(2) cells were transfected with control siRNA, N-Myc siRNA-1 or HDAC2 siRNA-2 for 48 hours. Protein was extracted from the cells and immunoprecipitated (IP) with an anti-p53 antibody. The immunoprecipitated protein was subjected to immunoblot analyses with anti-Ser-46-phos p53, anti-total p53 and anti-TP53INP1 antibodies. Error bars represent standard error. *** indicates P < 0.001.