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. 2014 May 20;5(12):4257–4268. doi: 10.18632/oncotarget.1991

Figure 4. Transcriptional repression of TP53INP1 protects neuroblastoma cells against apoptosis.

Figure 4

A, Kelly cells were transfected with control siRNA, TP53INP1 siRNA-1 or TP53INP1 siRNA-2. TP53INP1 mRNA and protein expression was analysed by real-time RT-PCR and immunoblot. B and C, Kelly cells were transfected with control siRNA, HDAC2 siRNA-1, HDAC2 siRNA-2, TP53INP1 siRNA-1, TP53INP1 siRNA-2, HDAC2 siRNA-1 plus TP53INP1 siRNA-1, or HDAC2 siRNA-2 plus TP53INP1 siRNA-2. Seventy-two hours later, the cells were incubated with Alamar blue for Alamar blue assays (B) or stained with FITC-conjugated Annexin V for flow cytometry analysis of apoptotic cells (C). Error bars represent standard error. *, ** and *** indicate P < 0.05, 0.01 and 0.001, respectively.