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. 2014 May 25;5(12):4283–4294. doi: 10.18632/oncotarget.2005

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Copyright: © 2014 Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Formalin-fixed, paraffin sections of renal cell carcinoma kidneys were probed with purified, rabbit anti-Nek1 primary antibodies, and detected by biotin-labeled secondary antibodies and the Vector ABC system for immunohistochemical analysis. At low magnification (25X) (A), Nek1 expression is markedly higher in the tumor (right side) compared to surrounding normal kidney (left side). Higher power magnification (40X) shows subcellular localization of Nek1 in normal tubular epithelial and glomerular cells (B), and clear cells (C). The nuclear punctate dots staining pattern was observed in 100X magnification (C, insert). Similar staining pattern (much more Nek1 expression in RCC than in surrounding normal kidney parenchyma) was observed in 5 individual tumor samples (all Stage 1 tumors).