Figure 2. Stiff substrate enhances voltage-gated Ca²⁺ channel currents in hippocampal neurons and PC12 cells.

(a) Representative whole-cell Ba²⁺ current recordings from DIV8–10 neurons on soft and stiff substrates. (b) Mean I-V relationship curves from recordings of 10 neurons from three preparations. (c) Representative whole-cell Ba²⁺ current recordings from PC12 neurons cultured for 8–10 days on soft and stiff substrates. (d) Mean I-V relationship curves. The number of cells recorded in each case is shown in parenthesis. *, p < 0.05 denotes difference in the current density at the same membrane potential.