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. Author manuscript; available in PMC: 2015 Aug 25.
Published in final edited form as: Dev Cell. 2014 Aug 25;30(4):479–487. doi: 10.1016/j.devcel.2014.06.011

Figure 3. Oxidative damages and ROS accumulation in LanCL1 ko brains.

Figure 3

(A) Representative images of ethidium fluorescence (Eth, red) show the accumulation of reactive oxygen species (ROS) in the cortex of 8-week LanCL1 ko brain. Bar:50μm.

(B) Immunostaining with anti-4-Hydroxynonenal (4-HNE, red) and anti-NeuN antibody (green) shows an increase in 4-HNE positive neurons in the 8-week LanCL1 ko cortex. Bar, 50μm.

(C and D) Western blots and quantification show an increase in the level of 4-HNE in the cortex of the 8-week LanCL1 ko brain. Error bars indicate SEM, **p=0.0033, n=5.

(E and F) Western blots and quantification show increase in the level of 4-HNE in LanCL1 cortical culture. Error bars indicate SEM, *p=0.0236, n=3.

(G) Quantification shows increase of malondialdehyde (MDA) in cortex of 8-week LanCL1 ko. Error bars indicate SEM, ***p=0.0003, n=7.

(H and I) Western blots with DNP antibody and quantification show increase of DNP levels in the cortex of 8-week LanCL1 ko cortex. Error bars indicate SEM, *p=0.0176, n=5.

(J) Immunostaining with 8-oxoG antibody shows an increase in 8-oxoG positive cells (green) in the 8-week LanCL1 ko cortex. Bar, 50μm. The insets show enlarged neurons. Bar, 5μm.

(K) JC-1 staining shows an increase in the fluorescence intensity of monomeric form of JC-1 (green) in LanCL1 ko cultures (DIV14). Bar, 50μm.

See also Figure S3