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Scheme of the Orco di construct. (A) Two Orco subunits are fused together and spaced by the 1-transmembrane protein SCN1B to grant for a correct orientation of both Orco subunits. The 3.6 kb construct was cloned into a pcDNA3.1(-) expression vector using Xho I and Hind III restriction sites. (B) Western blot of CHO cells showing the expression of Orco wt and Orco di. The numbers under wt and di protein lanes indicate the relative protein levels of expression.
