Table 2.
Comparison of the random amplified polymorphic DNA (RAPD) and pulsed field gel electrophoresis (PFGE) patterns, and insecticidal activity of B. thuringiensis strains.
| Strain | RAPD pattern | PFGE pattern | Mortality (%) 1 | |||||
|---|---|---|---|---|---|---|---|---|
| 250 mg/L | 25 mg/L | |||||||
| 24 h | 48 h | 72 h | 24 h | 48 h | 72 h | |||
| TT1 | D11 | PT11 | 6.83 ± 0.00 | 12.57 ± 10.93 | 19.00 ± 12.00 | 4.24 ± 3.71 | 11.91 ± 1.14 | 10.19 ± 4.81 |
| TT2 | D12 | PT12 | 6.90 ± 1.04 | 20.07 ± 7.28 | 22.72 ± 3.70 | 4.55 ± 3.94 | 5.28 ± 6.31 | 4.94 ± 4.28 |
| TT3 | D13 | PT13 | 2.66 ± 4.60 | 1.49 ± 1.43 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| TT4 | D14 | PT14 | 0.00 ± 0.00 | 0.00 ± 0.00 | 12.67 ± 21.94 | 3.33 ± 5.77 | 2.33 ± 4.04 | 4.62 ± 4.00 |
| TT5 | D15 | PT15 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 96.55 ± 5.98 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT6 | D16 | PT16 | 93.10 ± 5.98 | 96.36 ± 6.31 | 96.00 ± 6.93 | 86.20 ± 15.81 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT7 | D17 | PT17 | 96.86 ± 5.44 | 96.68 ± 5.74 | 96.33 ± 6.35 | 86.20 ± 5.98 | 96.33 ± 6.35 | 100.00 ± 0.00 |
| TT8 | D18 | PT18 | 86.20 ± 15.81 | 100.00 ± 0.00 | 100.00 ± 0.00 | 2.28 ± 3.94 | 16.33 ± 5.77 | 15.12 ± 6.68 |
| TT9 | D19 | PT19 | 96.55 ± 5.98 | 100.00 ± 0.00 | 100.00 ± 0.00 | 75.85 ± 5.98 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT10 | D20 | PT20 | 88.89 ± 11.51 | 100.00 ± 0.00 | 100.00 ± 0.00 | 57.04 ± 12.75 | 89.33 ± 0.58 | 92.63 ± 6.40 |
| TT11 | D21 | PT21 | 4.55 ± 3.95 | 30.78 ± 6.31 | 26.70 ± 6.68 | 2.28 ± 3.94 | 2.00 ± 0.00 | 0.00 ± 0.00 |
| TT14 | D24 | PT23 | ND 2 | ND | ND | ND | ND | ND |
| TT15 | D25 | PT24 | 93.10 ± 11.95 | 96.36 ± 6.31 | 96.00 ± 6.93 | 14.91 ± 20.19 | 38.00 ± 45.40 | 42.13 ± 40.09 |
| TT16 | D26 | PT25 | 30.99 ± 5.98 | 74.50 ± 12.62 | 76.85 ± 11.57 | 4.55 ± 3.94 | 5.67 ± 6.35 | 2.47 ± 4.28 |
| TT17 | D27 | PT26 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 79.30 ± 10.35 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT18 | D28 | PT27 | 96.17 ± 6.63 | 100.00 ± 0.00 | 100.00 ± 0.00 | 65.49 ± 33.28 | 96.33 ± 6.35 | 96.14 ± 6.68 |
| TT19 | D29 | PT28 | 4.55 ± 3.94 | 1.64 ± 0.00 | 2.47 ± 4.28 | 4.93 ± 4.31 | 17.01 ± 5.74 | 24.54 ± 9.62 |
| TT20 | D30 | PT29 | 86.20 ± 5.98 | 100.00 ± 0.00 | 100.00 ± 0.00 | 34.44 ± 5.98 | 74.33 ± 12.70 | 72.99 ± 13.36 |
| TT21 | D31 | PT30 | 68.94 ± 0.00 | 92.71 ± 6.31 | 92.00 ± 6.93 | 17.18 ± 0.00 | 38.07 ± 6.31 | 45.99 ± 6.68 |
| TT22 | D32 | PT31 | 89.65 ± 10.35 | 100.00 ± 0.00 | 100.00 ± 0.00 | 44.79 ± 5.98 | 92.67 ± 12.70 | 92.28 ± 13.36 |
| TT23 | ND | ND | 3.33 ± 5.57 | 11.51 ± 14.37 | 20.67 ± 6.35 | 3.33 ± 5.77 | 10.07 ± 6.26 | 20.10 ± 6.57 |
| TT24 | D33 | PT32 | 48.13 ± 3.23 | 89.66 ± 10.34 | 89.66 ± 10.34 | 3.33 ± 5.77 | 10.38 ± 5.97 | 13.82 ± 5.97 |
| TT25 | D34 | PT33 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 93.10 ± 11.95 | 96.33 ± 6.35 | 96.14 ± 6.68 |
| TT26 | D35 | PT34 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 93.33 ± 5.77 | 96.67 ± 5.77 | 96.49 ± 6.07 |
| TT27 | D36 | PT35 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 93.33 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT28 | D22 | PT36 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 89.27 ± 11.12 | 96.33 ± 6.35 | 96.11 ± 6.74 |
| TT29 | D34 | PT37 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 93.33 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT30 | D37 | PT38 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 93.33 ± 11.55 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT31 | D38 | PT39 | 96.67 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | 90.00 ± 17.32 | 96.67 ± 5.77 | 96.49 ± 6.07 |
| TT32 | D22 | PT40 | 93.33 ± 11.55 | 100.00 ± 0.00 | 100.00 ± 0.00 | 62.77 ± 6.32 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT33 | D39 | PT41 | 96.67 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | 71.20 ± 8.27 | 90.33 ± 0.58 | 89.80 ± 0.55 |
| TT34 | D40 | PT41 | 93.33 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | 73.33 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT35 | D38 | PT42 | 63.33 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | 21.67 ± 2.89 | 77.67 ± 10.97 | 87.94 ± 13.01 |
| TT36 | D41 | PT43 | 90.00 ± 10.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 10.00 ± 0.00 | 44.85 ± 5.97 | 58.63 ± 10.34 |
| TT37 | D42 | PT44 | 80.00 ± 34.64 | 100.00 ± 0.00 | 100.00 ± 0.00 | 46.67 ± 5.77 | 96.67 ± 5.77 | 96.55 ± 5.97 |
| TT38 | D43 | PT45 | 80.00 ± 26.46 | 100.00 ± 0.00 | 100.00 ± 0.00 | 33.33 ± 11.55 | 72.33 ± 11.55 | 72.42 ± 11.94 |
| TT39 | D44 | PT46 | 96.67 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | 33.75 ± 5.30 | 76.73 ± 3.66 | 76.73 ± 3.66 |
| TT40 | D36 | PT47 | 90.00 ± 17.32 | 100.00 ± 0.00 | 100.00 ± 0.00 | 50.00 ± 43.59 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT41 | ND | ND | 10.00 ± 10.00 | 17.00 ± 0.00 | 8.00 ± 6.93 | 6.67 ± 5.77 | 6.93 ± 0.00 | 0.00 ± 0.00 |
| TT42 | D34 | PT48 | 96.67 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | 43.33 ± 5.77 | 86.33 ± 15.82 | 92.03 ± 6.91 |
| TT43 | D45 | PT49 | 13.33 ± 5.77 | 37.95 ± 10.34 | 40.19 ± 11.96 | 0.00 ± 0.00 | 4.62 ± 4.00 | 2.87 ± 2.49 |
| TT44 | D46 | PT50 | 40.00 ± 0.00 | 58.63 ± 10.34 | 52.00 ± 12.00 | 3.33 ± 5.77 | 20.72 ± 5.97 | 12.28 ± 6.91 |
| TT45 | D47 | PT50 | 96.67 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | 83.33 ± 5.77 | 96.67 ± 5.77 | 96.01 ± 6.91 |
| TT46 | D33 | PT51 | 90.00 ± 10.00 | 96.55 ± 5.87 | 96.00 ± 6.93 | 60.00 ± 10.00 | 79.33 ± 10.50 | 80.06 ± 13.81 |
| TT47 | D43 | PT52 | 96.67 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | 74.53 ± 9.47 | 84.00 ± 10.39 | 83.42 ± 10.50 |
| TT48 | D43 | PT52 | 93.33 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | 60.00 ± 10.00 | 82.67 ± 6.35 | 88.04 ± 11.96 |
| TT49 | D43 | PT52 | 88.57 ± 2.48 | 100.00 ± 0.00 | 100.00 ± 0.00 | 80.00 ± 10.00 | 96.67 ± 5.77 | 96.01 ± 6.91 |
| TT50 | D43 | PT52 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 83.33 ± 15.28 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT51 | D48 | PT1 | 70.00 ± 10.00 | 96.55 ± 5.97 | 96.00 ± 6.93 | 3.33 ± 5.77 | 24.33 ± 6.35 | 24.24 ± 6.91 |
| TT52 | D38 | PT53 | 96.67 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | 54.53 ± 7.85 | 84.33 ± 13.80 | 82.06 ± 15.82 |
| TT53 | D41 | PT54 | 96.61 ± 5.87 | 100.00 ± 0.00 | 100.00 ± 0.00 | 86.45 ± 5.87 | 89.33 ± 10.50 | 88.91 ± 11.09 |
| TT54 | D2 | PT2 | 96.61 ± 5.87 | 100.00 ± 0.00 | 100.00 ± 0.00 | 39.02 ± 0.00 | 47.42 ± 10.52 | 51.96 ± 6.40 |
| TT55 | D49 | PT55 | 10.00 ± 10.00 | 17.27 ± 10.34 | 20.72 ± 5.97 | 3.33 ± 5.77 | 7.00 ± 0.00 | 13.82 ± 5.97 |
| TT56 | D50 | PT56 | 0.00 ± 0.00 | 3.58 ± 3.10 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.07 ± 0.13 |
| TT57 | D51 | PT57 | 6.54 ± 5.80 | 4.45 ± 4.07 | 1.00 ± 1.73 | 2.85 ± 4.93 | 3.58 ± 3.10 | 0.15 ± 0.13 |
| TT58 | D22 | PT58 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 89.84 ± 10.16 | 96.33 ± 6.35 | 96.30 ± 6.40 |
| TT59 | D38 | PT59 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT60 | D52 | PT60 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 96.61 ± 5.87 | 96.33 ± 6.35 | 96.30 ± 6.40 |
| TT61 | D34 | PT61 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 96.61 ± 5.87 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT62 | D53 | PT62 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 |
| TT12 (positive control) | D22 | PT17 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | 79.30 ± 10.35 | 96.33 ± 6.35 | 96.14 ± 6.68 |
| TT13 (positive control) | D23 | PT22 | 96.55 ± 5.98 | 100.00 ± 0.00 | 100.00 ± 0.00 | 50.17 ± 3.35 | 81.33 ± 12.42 | 80.29 ± 13.01 |
| Tris buffer (negative control) | - | - | 1.00 ± 3.05 | 4.67 ± 5.71 | 9.27 ± 7.31 | 1.00 ± 3.05 | 4.67 ± 5.71 | 9.27 ± 7.31 |
1 All data were expressed as mean ± standard deviation (n = 3); 2 ND: Not determined.