Table 3.
Comparison of cry gene profiles and insecticidal activity of B. thuringiensis strains.
| Strain | Mortality (%) 1 | cry genes 2 | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 25 mg/L (global Cry protein) | ||||||||||||||||
| 24 h | 48 h | 72 h | 1 | 1B | 1E | 2Aa1 | 3A | 4A2 | 7 | 8 | 9A | 9C | 9Ea | 22 | 32Aa | |
| TT59 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | + | - | - | + | - | - | - | - | - | - | - | - | - |
| TT62 | 100.00 ± 0.00 | 100.00 ± 0.00 | 100.00 ± 0.00 | + | - | - | + | - | - | - | - | - | - | - | - | - |
| TT61 | 96.61 ± 5.87 | 100.00 ± 0.00 | 100.00 ± 0.00 | + | - | - | + | - | - | - | - | - | - | - | - | - |
| TT5 | 96.55 ± 5.98 | 100.00 ± 0.00 | 100.00 ± 0.00 | + | - | - | - | - | - | - | - | - | - | + | - | - |
| TT27 | 93.33 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | + | - | - | + | - | - | - | - | - | - | - | - | - |
| TT29 | 93.33 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | + | - | - | + | - | - | - | - | - | - | - | - | - |
| TT30 | 93.33 ± 11.55 | 100.00 ± 0.00 | 100.00 ± 0.00 | + | - | - | - | - | - | - | - | - | - | + | - | - |
| TT25 | 93.10 ± 11.95 | 96.33 ± 6.35 | 96.14 ± 6.68 | + | - | - | + | - | - | - | - | - | - | - | - | - |
| TT50 | 83.33 ± 15.28 | 100.00 ± 0.00 | 100.00 ± 0.00 | + | + | - | + | - | - | - | - | - | - | - | - | - |
| TT49 | 80.00 ± 10.00 | 96.67 ± 5.77 | 96.01 ± 6.91 | + | + | - | + | - | - | - | - | - | - | - | - | - |
| TT17 | 79.30 ± 10.35 | 100.00 ± 0.00 | 100.00 ± 0.00 | + | - | - | + | - | - | - | - | - | - | - | - | - |
| TT12 | 79.30 ± 10.35 | 96.33 ± 6.35 | 96.14 ± 6.68 | + | - | - | + | - | - | - | - | - | - | - | - | - |
| TT47 | 74.53 ± 9.47 | 84.00 ± 10.39 | 83.42 ± 10.50 | + | + | - | + | - | - | - | - | - | - | - | - | - |
| TT34 | 73.33 ± 5.77 | 100.00 ± 0.00 | 100.00 ± 0.00 | + | - | - | + | - | - | - | - | - | - | - | - | - |
| TT48 | 60.00 ± 10.00 | 82.67 ± 6.35 | 88.04 ± 11.96 | + | + | - | + | - | - | - | - | - | - | - | - | - |
| TT41 | 6.67 ± 5.77 | 6.93 ± 0.00 | 0.00 ± 0.00 | + | - | - | + | - | - | - | - | - | - | - | - | - |
| TT19 | 4.93 ± 4.31 | 17.01 ± 5.74 | 24.54 ± 9.62 | + | - | - | - | - | - | - | - | - | - | - | - | - |
| TT2 | 4.55 ± 3.94 | 5.28 ± 6.31 | 4.94 ± 4.28 | + | - | - | - | - | - | - | - | - | - | - | - | - |
| TT16 | 4.55 ± 3.94 | 5.67 ± 6.35 | 2.47 ± 4.28 | + | - | - | - | - | - | - | - | - | - | - | - | - |
| TT1 | 4.24 ± 3.71 | 11.91 ± 1.14 | 10.19 ± 4.81 | + | - | - | + | - | - | - | - | - | - | - | - | - |
| TT44 | 3.33 ± 5.77 | 20.72 ± 5.97 | 12.28 ± 6.91 | + | - | - | - | - | - | - | - | - | - | + | - | - |
| TT24 | 3.33 ± 5.77 | 10.38 ± 5.97 | 13.82 ± 5.97 | + | - | - | + | - | - | - | - | - | - | - | - | - |
| TT4 | 3.33 ± 5.77 | 2.33 ± 4.04 | 4.62 ± 4.00 | + | - | - | - | - | - | - | - | - | - | - | - | - |
| TT23 | 3.33 ± 5.77 | 10.07 ± 6.26 | 20.10 ± 6.57 | + | - | - | - | - | - | - | - | - | - | - | + | - |
| TT57 | 2.85 ± 4.93 | 3.58 ± 3.10 | 0.15 ± 0.13 | + | - | - | - | - | - | + | - | - | - | - | - | - |
| TT11 | 2.28 ± 3.94 | 2.00 ± 0.00 | 0.00 ± 0.00 | + | - | - | - | - | - | - | - | - | - | - | - | - |
| TT43 | 0.00 ± 0.00 | 4.62 ± 4.00 | 2.87 ± 2.49 | + | - | - | - | - | - | - | - | - | - | - | - | - |
| TT56 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.07 ± 0.13 | + | - | - | - | - | - | + | - | - | - | - | - | - |
| TT3 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | + | - | - | - | - | - | - | - | - | - | - | - | - |
| Total | 29+ 3 | 4+ | 0+ | 16+ | 0+ | 0+ | 2+ | 0+ | 0+ | 0+ | 3+ | 1+ | 0+ | |||
1 All data were expressed as mean ± standard deviation (n = 3); 2 The presence (+) or the absence (-) of the specific cry gene by PCR was shown; 3 Number of strains positive for specific cry gene.