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. 2014 Aug 19;5(4):e01116-14. doi: 10.1128/mBio.01116-14

FIG 1 .

FIG 1 

LPS modification in E. coli biofilm bacteria. (A) Tricine SDS-PAGE/periodate-silver staining analysis of LPS extracted from planktonic (Pk) or biofilm (Bf) E. coli K-12 BW25113. The arrows indicate a modified LPS band. To assess whether biofilm bacteria reinoculated in planktonic conditions still display a modified LPS profile, bacteria grown for 96 h were recultured overnight in planktonic conditions (Bf → Pk). (B) LPS analysis of 24-h planktonic or 96-h biofilm cultures from pathogenic E. coli strains, including enteroaggregative E. coli (EAEC) strains 55989 and O42 and uropathogenic E. coli (UPEC) strains 536 and CFT073. (C) Comparison, at different time points, of biofilm biomass produced in continuous-flow microfermentors by E. coli K-12 BW25113 strain with its closely related derivative BW25113 F, carrying the biofilm-promoting F conjugative plasmid. O.D.600, optical density at 600 nm. (D) Tricine SDS-PAGE/periodate-silver staining analysis of LPS extracted from corresponding biofilm cultures.