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. 2014 Aug 20;7:122. doi: 10.1186/s13068-014-0122-x

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© Lee et al.; licensee Springer 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Rational engineering of S. cerevisiae expressing a xylose isomerase pathway. Rational strain engineering in a gre3 background containing an integrated copy of xylA3* and XKS1 overexpression (SXA-R1 strain) was evaluated. In addition, the expression of an additional copy of xylA3* and tal1 overexpression (square), and pho13 deletion (black) gradually improved the cell growth on xylose. Strain identifications (SXA-R1: white circle, SXA-R2: white square, SXA-R1P: black circle, and SXA-R2P: black square) are described in the text. The expression of an additional copy of xylA3* and pho13 deletion showed synergistic effect on the cell growth on xylose. Error bars represent the standard deviation of biological triplicates.