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. 2014 Aug 20;7:122. doi: 10.1186/s13068-014-0122-x

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© Lee et al.; licensee Springer 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Adaptive evolution of the rationally engineered strain expressing the xylose isomerase pathway. The adaptive evolution of the rationally engineered strain (SXA-R2P) was performed in the exponential cell growth phase transfer set with different inoculum sizes of low (0.5%: black), medium (1%: grey), and high (5%: white). The graph shows representative strains with improved cell growth on xylose. The strains adapted in the culture with low inoculum size showed the highest improvement in the cell growth.