Table 3.
TaqMan primer sets for ANKRD6 gene SNPs examined in this study.*
| SNP | RefSeq No. | Forward primer | Reverse primer | WT allele probe (5′ VIC) | MT allele probe (5′ FAM) |
|---|---|---|---|---|---|
| 233 T>M | rs2273238 | AAGGTGGCCAAAATCTTACTGGAA | TGGGAACCACTTTAGAAAGCAATGT | CAATGGTCGTATCTGC | CAATGGTCATATCTGC |
| 631 P>L | rs61739327 | GGGAAGAGTGGGCCAACAAG | GCCCACAGGTGCTGCT | CTGCGGGTTGCTG | CTGCGAGTTGCTG |
| 122 Q>E | rs16881983 | AGCCTTGCATGAAGCATCCT | CGTTGGCTCCTGCTTTAATGAG | CTGACTGGCTGAAAC | CTGACTCGCTGAAAC |
| 128 I>L | rs3748085 | GGCATGGTTTCAGCCAGTCA | CTTGTTCTTGGCAAGCACGTT | CAAGCTGCTCATTAAA | AAGCTGCTCGTTAAA |
| 710 L>X | NOVEL | CCCAGCAAGATAAGGCTACATTGAA | CCCTAGTCCTTAGTTTGGCAAGTT | CCTCTTCTAAACTTTTAATGTG | TCTTCTAAACTTTAAATGTG |
| 197805 G>A | rs9344950 | GGACTAGGGTGCAGAAGGAAAATTA | GCAAAACTGGAAATCTTCAAGAATCCT | CACCAATAAAGAGGAAAT | CACCAATAAAAAGGAAAT |
| 485 M>L | rs61736690 | GGGCTCACCCTGAGATCATCT | CTCAACCATCAGCTTGTCCAAAA | CTTCACTCCTAGATGCGTG | TTCACTCCTAGCTGCGTG |
| 545 T>A | rs9362667 | ACCAGGTGTGGACCAATTAGTG | CCTTCTCTTTGGGCCTAACCA | CAGCAGCAGCTTC | CAGCAACAGCTTC |
*
ANKRD6 = Ankyrin Repeat Domain 6; SNP = single-nucleotide polymorphisms; VIC = VIC® (dye labeled probe); FAM = FAM™ (dye labeled probe).