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. 2014 Aug 21;14:224. doi: 10.1186/s12870-014-0224-4

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© Rodríguez-Sanz et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunolocalization of 5mdC, esterified (JIM7) and non-esterified (JIM5) pectins, and IAA in other systems. A-E: Zygotic torpedo embryo of Brassica napus. F-K: Mature anther of Quercus suber. A, F: Panoramic views of zygotic torpedo embryo and mature anther, resin sections stained by toluidine blue; squares in A and F indicate representative regions of proliferating embryo cells (A) and differentiated cells of anther wall (B) where the analysis is focused. B-E, G-K: Higher magnifications of immunofluorescence signals (green) for 5mdC (B, G), JIM5 (C, H), JIM7 (D, J) and IAA (E, K) antibodies. In B, DAPI staining for nuclei (blue) is merged with 5mdC immunofluorescence (green). Insets in B and G show representative nuclei of each cell types, at high magnification. Bars: A, F: 50 μm, B-E, G-K: 25 μm, insets: 5 μm.