Table 2.
Marker | TA (°C) | Chromosome | Forward (5′–3′) | Reverse (5′–3) |
---|---|---|---|---|
Li22-35 | 52 | 1 | CTTGATGTTCGGGTTAGCAAG | ATGCACACCAAAAATCATGTG |
Li41-56 | 50 | 36 | TTGCTTCATGATAACAACTTGG | CCTGTTGGTGTGAGTTCGTG |
Li46-67 | 50 | 31 | TCTTCTTTCGTTAGCTGAGTGC | CTGTATCACCCATGAGGGGC |
Li71-7 | 50 | 30 | GCTGCAGCAGATGAGAAGG | GTGAGAAGGCAGGGATTCAA |
Li71-33 | 50 | 31 | CTCCTTTCACACCCGCCTCT | GAGAGAAGACGAGCCGAAGT |
TA, temperature of annealing.